earticle

영어

Deracemization is an important tool for the industrial production of single enantiomeric compounds. It has a significant advantage over the kinetic resolution approach by allowing the complete recovery of a single enantiomer from the racemic mixtures. In this study, we have developed a novel approach for deracemization through the combination ω-transaminase and DAAO with improved oxygen usage by the help of a hemoglobin fused DAAO form. This combination of D-selectivity of amino acid oxidase with ω-transaminase has been applied for deracemization of the racemic mixture of 2-amino butyric acid, a non-natural amino acid and some drug candidates. The catalytic activity of D-amino acid oxidase (DAAO) was enhanced by improving the oxygen usage of DAAO by fusing it with Vitreoscilla hemoglobin (VHb) which helps the binding of free oxygen. Using this approach, we successfully obtained a quantitative yield of L-2-aminobutyric acid from D, L-2-aminobutyric acid. This novel approach can help for the scalable production of valuable compounds by deracemization from the racemic mixtures