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포스터 발표 : 효소공학

Heterologous expression of Phanerochaete chrysosporium Manganese Peroxidase isoenzyme H4 in the yeast Pichia pastoris

원문정보

Seung-Moon PARK, Saravana KUMAR, Ae-Young MO, Yu-Lim SON, Dae-Hyuk KIM, Jung-Chae LEE, Seung-Moon PARK

한국생물공학회 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회 2011.04 pp.176-176
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Lignin biodegradation is a key step for carbon recycling in terrestrial ecosystems, where white-rot basidiomycetes fungi degrade this recalcitrant wood polymer enabling cellulose utilization by microbial populations. Three peroxidases involved in lignin degradation are known as lignin peroxidase (LiP), manganese peroxidase (MnP), versatile peroxidase (VP). The cDNA encoding manganese peroxydase isozyme H4 (MnPH4), isolated from Phanerochaete chrysosporium, was cloned into the pPICZα vectors and expressed in Pichia pastoris under the control of the methanol inducible alcohol oxidase I (AOXI) promoter. Enzyme assay indicated that the recombinant MnPH4 is efficiently secreted into the medium upon hemoglobin supplementation, at a maximum concentration of 500 U l-1. The purified recombinant manganese peroxidase (rMnP) exhibited a 60 kDa molecular mass considerably larger, because of their glycosylation, and showed optimum pH of 4.5 and temperature at 35 oC.

키워드

저자정보

  • Seung-Moon PARK Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Saravana KUMAR Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Ae-Young MO Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Yu-Lim SON Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Dae-Hyuk KIM Research Center for Bioactive Materials, Chonbuk National University, Jeonju, 561-756, Korea.
  • Jung-Chae LEE Research Center for Bioactive Materials, Chonbuk National University, Jeonju, 561-756, Korea.
  • Seung-Moon PARK Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.

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자료제공 : 네이버학술정보

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