원문정보
초록
영어
Stability and activity of immobilized forms of Candida antarctica lipase B and Thermomyces lanuginosus lipase were studied for enzymatic biodiesel production in a non-solvent system at atmospheric pressure and in supercritical carbon dioxide (ScCO2). The lipases immobilized by several methods(entrapment in alginate and silica sol-gel, cross-linking, adsorption, and covalent binding) as well as commercial immobilized enzymes(Novozym 435 and Lipzyme TL IM from Novozymes) were used for a comparative study. The model reaction used was the transesterfication of canola oil with methanol. From the experimental result, it was found that among tested enzymes Lipozyme TL IM and the Thermomyces lanuginosus lipase immobilized in silica sol-gel were most stable under the reaction conditions and the conversion could be enhanced by using ScCO2. These results should help in determining suitable biocatalysts for the enzymatic biodiesel production.