원문정보
초록
영어
With the aim of isolating a microbial biocatalyst with potential application in the field of bio-fuel, a lipase from actinomycetes strain recently isolated from Korean soil, was purified using sequential chromatographic steps. Molecular weight of the enzyme was estimated to be 32,400 Da by SDS-PAGE. The enzyme was highly active at low temperature with maximum at 30°C and pH 8.0. The activity at 3°C was about 43% of the maximum activity, thus the enzyme showed the characteristics of low temperature-active enzymes. All the tested detergents, except Triton X-100, inhibited the enzyme activity by various extents. The enzyme preferentially hydrolyzed p-nitrophenyl deconate, a medium chain substrate. More importantly, it catalyzed biodiesel production using palm oil and methanol. The enzyme thus could be potentially applicable in bio-fuel industry.