원문정보
초록
영어
One Streptomyces strain, which was recently isolated from Korean soil, produced extracellular lipase in the OSYM culture medium. The enzyme was purified using a single step gel permeation chromatography on Sepharose CL-6B. The enzyme was nearly homogenous in SDS-PAGE analysis with a single band. The activity was optimum at 40 °C and pH 7.0 and was stable between pH 5.0 and 8.0 and below 50 °C. Most of the tested metal ions as well as detergents showed inhibitory effect on the enzyme activity, but with various extents.. The enzyme showed highest hydrolytic activity with p-nitrophenyl palmitate (pNPP), a long chain substrate and Km and Vmax of the enzyme for the substrate were determined to be 0.24 mM and 4.6 mM/min mg, respectively. Furthermore, the enzyme hydrolyzed triolein producing 1,2- and 1,3-diolein along with oleic acid. More importantly, it catalyzed biodiesel production in presence of methanol and various oils; therefore, it can be applicable in bio-energy industry.