earticle

영어

Conversion of cellulose to glucose is the most critical step for utilization of cellulose as biomass. Enzymatic hydrolysis of cellulose is achieved by cooperation of three types of cellulases including endoglucanase, exoglucanase, and β-glucosidase. Recently, yeast strains which are engineered to express cellulases have been developed to improve bioethanol production from lignocellulosic biomass. In this study, we focused on searching for endoglucanases that possess sufficient activity when expressed on yeast surface. We expressed various endoglucanases from fungi and bacteria on the surface of Sacchoromyces cerevisiae, and the activities of these recombinant enzymes were determined at various temperature conditions using Carboxymethylcellulose (CMC). As a result, Clostridium thermocellum CelD and Thermoascus aurantiacus endoglucanase I showed enzyme activities comparable with that of Trichoderma reesei endoglucanase II, which is widely used for bioethanol production studies. Therefore, these novel endoglucanases might be useful to generate yeast strains for consolidate bioprocessing of lignocellulosic biomass to ethanol.