earticle

영어

A capnophilic rumen bacteria, Actinobacillus succinogenes can produce high level of succinic acid as a major fermentation end product. Succinic acid is a four-carbon dicarboxylic acid, produced as an intermediate of the tricarboxylic acid cycle and can be used to produce various industrially useful chemicals like 1,4-butandiol and tetrahydrofuran, as well as biodegradable polymers. During the anaerobic fermentation, the carbon dioxide availability is a key factor determining the succinic acid productivity. In this study, to increase the succinic acid productivity, optimization of the CO2 supply method and concentration of the magnesium carbonate (MgCO3) as CO2 source of the media was performed. In this case, batch-type fermentation is problematic because of high requirement and low solubility of the magnesium carbonate, especially in early phase of the fermentation. Therefor in fed-batch mode, the magnesium carbonate feeding time and the concentration were controlled according to the cell growth during the fermentation to maximize the succinic acid productivity. It was found that total magnesium carbonate requirement is lower in fed-batch fermentation than in batch culture, providing a way of cost reduction.