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영어

Carrageenans are classified according to the number and the position of sulfate ester groups. They are made up of linear chains of galactose. The κ-carrageenase CgkA from Pseudoalteromonas carrageenovora is a hydrolase involved in the degradation of carrageenan. In this study, we expressed recombinant scaffolding protein mini-CbpA from Clostridium cellulovorans in Escherichia coli. To assemble the carrageenan-degrading complex via cohesin-dockerin interaction, a chimeric carrageenase cCgkA was constructed to containing the catalytic domain of CgkA fused with a dockerin domain of C. cellulovorans EngB. The resulting hydrolysis complex could degrade carrageenan efficiently with synergic effect of carrageenan-degrading enzyme activities. This is the first report on the formation of carrageenan-degrading complex using the cohesin-dockerin interaction system. The enhancement of carrageenandegrading complex activities will lead to the commercial production of useful products from agar biomass at low costs. [This Study was supported by Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea(No. 309016-5)]