원문정보
초록
영어
In human intestinal tract is one of the most varied and complex ecosystems, and dietary life an important role in the presence and prevalence of probiotics (lactobacilli and bifidobacterium). The composition and dynamics of fecal microbial communities during the study were analyzed by the PCR-denaturing gradient gel electrophoresis technique using group and genus-specific primers. DGGE of fecal 16S rDNA amplicons from 6 volunteer individuals showed host-specific populations of lactobacilli and bifidobacterium that were stable over a period of 5 weeks. Group specific Lactobacilli such as Lactobacillus sp., Leuconostoc sp., and Weisella sp. and genus specific bifidobacterium such as B. longum, B. coryneforme, B. adolescentis, B. breve, and Bacteroides fragilis were detected in human feces. These results indicate that the community of Lactobacilli and Bifidobacterium in human feces is unique because of the presence of specific species for human feces and a minority of the Lactobacilli and Bifidobacterium group may be caused by dietary life. In the future, intestinal tract microbial research can be regarded as a lactic acid bacteria-rich food providing various probiotics.