원문정보
초록
영어
This study is focused on analyzing the enhanced hydrogen production by extracted mixed organic compounds from Rhodobacter sphaeroides. In this study, recombinant plasmid was constructed to analyze the effect of hydrogen production by the expression of HupSL hydrogenase isolated from R. sphaeroides in Escherichia coli. Hydrogen production by expression of HupSL hydrogenase from recombinant E. coli was increased 20.9-fold compared to control E. coli. In addition, we found that biohydrogen production from recombinant E. coli was more increased under the mixed organic compounds supplemented condition. The concentration of the ATP as cofactor for hydrogen production from in mixed organic compounds extracted from R. sphaeroides showed about 10-7M, while E. coli extract was about 10-10M. From these results, we conclude that mixed organic compounds from R. sphaeroides have multifunctional potential to increase the fermentation efficiency of microorganism.