원문정보
초록
영어
Biohydrogen production can be one of solutions for energy crisis. Previously, we successfully demonstrated the production of biohydrogen by constructing [NiFe]-hydrogenase-expressing recombinant E. coli. Although [NiFe]-hydrogenase showed relatively high oxygen-tolerance compare to other hydrogenases, still biohydrogen production efficiency was low. In this research, we introduced proteorhodopsin, light acceptor for prokaryote working as proton pump, into [NiFe]-hydrogenaseexpressing recombinant E. coli for utilizing light energy for producing biohydrogen. Proteorhodopsin need a chemical named retinal for working as proton pump by using light energy but E. coli does not have a pathway to synthesize it. In order to give E. coli an ability to synthesize retinal, we expressed five foreign genes expressing enzymes for retinal synthesis pathway. By introducing light accepting system into E. coli expressing [NiFe]-hydrogenase, we could get enhanced biohydrogen productivity.