earticle

영어

Citrus peel waste is produced after the extraction of juice, from citrus fruits. The citrus peel waste contains about 27% solid while a 73% of liquid content. The juicy content was used as a substrate for fermentation to produce antimicrobial substance. The organism used for fermentation was Bacillus sp. LS 1-2, isolated from Korean transitional soybean paste foods. It was confirmed as Bacillus sp based on 16S rRNA sequence similarities. LS 1-2 was cultured in citrus-processing-waste containing five Brix as a culture medium and produced an antimicrobial agent against both gram-positives and negatives at adequate culture conditions (pH, Temperature, Culture period and, etc.). An antimicrobial agent produced by LS 1-2 was purified by the consecutive steps of the alcohol precipitation, C-18 cartridges, Recycle preparative HPLC and preparative HPLC. The active compound was not sensitive to lipase, proteinase K, amylase, catalase but was affected by pronase E. In order to evaluate its commercial value, the minimum inhibitory concentration of purified antim icrobial agent from Bacillus sp. LS 1-2 was compared with commercialized antimicrobial agents (Ampicillin) against Staphylococcus aureus and Escherichia coli O-157.