earticle

영어

In this study, we report the structure and shape of PS-PSMA nanofibers with entrapped QDs, that were prepared by electrospinning a homogenous mixture of polymers and QDs. Uniformly distributed QDs within the polymer matrix of nanofibers induced high degree of compactness and shape rigidity in the nanofibers and also showed efficient enzyme immobilization. The esterase-nanofiber composites were prepared by fabricating crosslinked enzyme aggregates on the surfaces of nanofibers. After immobilization, the Vmax value of the QD embedded esterasenanofibers system was higher compared to pristine nanofibers. Furthermore, the esterase enzyme coating on the QDs nanofibers retained good stability with no loss of enzyme activity even after recycling for more than ten times. The present study demonstrates the successful application of QDs PS-PSMA nanofibers for enzyme immobilization for the first time; it is anticipated that this technology would be employed in various enzyme applications due to the facile shape control of the nanofibers.