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영어

In this study, we degraded several toxic chemicals, which can be released from the pretreatment of lignocellulose for the biofuel production, using microorganisms isolated from the soil. First, the strains were characterized with growth on pure hydrolysate compounds including p-coumaric acid and vanilic acid within media. All strains grew most quickly on 4-hydroxy benzoic acid having the simplest chemical stucture. We also performed the fed-batch experiment and checked the maximum degradation. Finally, we confirmed the ability of these isolates to mineralize those chemicals using E. coli biosensor. In our previous study, we constructed a bioluminescent fusion strain and demonstrated that the strain have the potential as a biosensor to analyze phenolics present in hydrolysate sample. As shown, bioluminescence responses decreased according to time, suggesting that the concentration of chemicals in media also decreased.