earticle

영어

The purpose of this study was to investigate the relationships between hydrolysis of particulate organic materials (POM) and microorganisms in acidogenic reactors treating food-waste recycling wastewater (FRW). Hydrolysis efficiency in terms of volatile suspended solid (VSS) reduction was observed in continuous completely stirred tank reactors (CSTRs) with changing hydraulic retention time HRT (1 - 3 day), pH (4 - 6), and temperature (25 - 45oC) and was interpreted by response surface methodology (RSM). The maximum hydrolysis efficiency was estimated as 47% reduction of VSS when (HRT), pH and (T) were at 3 days, 6.0 and 45℃, respectively. Denaturing gel gradient electrophoresis (DGGE) using bacterial 16S rRNA genes were carried out to investigate acidogenic bacterial community structure. The ten identified acidogenic bacterial microorganisms (ABMs) were selected as target for developing speciesspecific primers and probe set for use of real time quantitative polymerase chain reaction (QPCR). The results of QPCR showed that Lactobacillus amylovorus, L. acetotolerans, and Bifidobacterium thermophilum had a large influence in the hydrolysis of POM.