원문정보
초록
영어
Gliadin, component of gluten, is known as a important allergen which cause allergic reactions to patients who have wheat allergy. This study was conducted to evaluate the effect of physical treatments on the antigenicity of gliadin in wheat flour and dough. The wheat flour was treated with supercritical carbon dioxide (SC-CO2) and in combination with autoclave and microwave. The conformational changes were observed by SDS-PAGE and the binding ability of gliadin to anti-gliadin IgG was examined by ci-ELISA and immunoblotting. The binding ability of wheat flour treated with SC-CO2 was slightly decreased compared with untreated flour. When treated with SC-CO2 and autoclave, it was shown that the binding ability to anti-gliadin IgG was decreased and the intensity of gliadin bands were weakened in SDS-PAGE. In case of the microwave treatment after SC-CO2 treatment, the binding ability was slightly reduced. When treated with SC-CO2, autoclave and microwave, the binding ability of gliadin was also decreased.