earticle

영어

A novel simultaneous microwave-assisted extraction (MAE) and adsorbent treatment process was developed for recovering the anticancer agent paclitaxel from plant cell cultures and removing the plant-derived tar and waxy compounds. Furthermore, the process efficiency was maximized by optimizing the major process variables (extraction time, amount of absorbent, biomass-to-methanol ratio, extraction temperature). The optimum conditions of the simultaneous process for efficiency of the recovery of paclitaxel and the removal of tar and waxy compounds were 6 min for the extraction time, 4.5 (w/v) for the ratio of adsorbent to crude extract, 1:1 (w/v) for the biomass-to-methanol ratio, and 40℃ for the extraction temperature. Performing the simultaneous process under the optimum conditions could recover most (>99%) of the paclitaxel with the purity of ~22% could be recovered from hexane precipitation which was the following process. This result was similar to the efficiency (purity: ~20%, yield: ~95%) of the conventional process in which the paclitaxel recovery process is separated from the adsorbent treatment process. Therefore, it is expected that the conventional process could be simplified by simultaneously performing the recovery of paclitaxel and the removal of plant-derived tar and waxy compounds from plant cell cultures as one process, thus dramatically reducing the cost of the paclitaxel separation/purification process