earticle

영어

Unicellular green algae, Haematococcus lacustris is able to produce astaxanthin up to 80% of dry cell weight, and it is induced under various stress conditions, such as high irradiance, high salinity, nutrient starvation, and oxidative stress by reactive oxygen species (ROS) as a defense mechanism of the cells. In this study, we used proteomic and transcriptomic analyses to obtain gene expression profiles in H. lacustris under astaxanthin-inductive culture conditions, combining high irradiance and nutrient starvation. In the experiments, we analyzed several genes that were differentially expressed more than twofold change. As the results, significant decrease in the expression of chlorophyll biosynthesis and light harvesting complex (LHC) related genes were shown in astaxanthin-accumulated H. lacustris (red cyst cells), that can decrease photosynthesis. On the other hand, respirationrelated genes, lipid metabolism-related genes and stress response-related genes were activated in the red cyst cells. These results provide comprehensive information for understanding cell responses during astaxanthin accumulation by stress conditions in H. lacustris.