earticle

영어

Nanosensors functionalized with biomolecules hold promise for many potential applications in biotechnology and nanomedicine. Biolumenescent nanosensors have been drawing much attention for in vitro and in vivo monitoring due to their extremely low background and high sensitivity. Our developed nanosensor is based on the energy transfer between the donor bioluminescent protein (luciferase) and its counterpart (nanoparticle), which was applied for the detection of matrix metalloproteinase (MMP) activity. MMP was chosen as the model protease because of its important role in promoting tumor progression and invasion. In this talk, emphasis will be made upon the broad applications of BL nanosensors to in vitro diagnosis and in vivo imaging with functional conjugation methods between biomolecules and nanoparticles. This approach will help expand the utility of bioluminescent detection in biology and nanotechnology research.