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Glycation of collagen causes skin yellowing via a spontaneous reaction among protein and sugar in the presence of reactive oxygen. Advenced glycosylation end products(AGEs) are produced by attaching an aldehyde group of glucose to amino group of a collagen molecule. These AGEs are yellowish in color and contribute to the yellow or sallow appearance of skin.(1-4) We know that fruit extracts contain a lot of antioxidant compounds and functional cosmetic raw materials. So we studied the effect of gold kiwifruit as anti aging agent and glycation inhibitor in collagen. We screened for in vitro antioxidant and anti-glycation activity of whole kiwifruit extract, kiwi pulp and rind extract. Rind portion exhibited more effective than pulp and whole kiwi extract in result. Thus it was selected to be further fractionated hexane, chloroform, ethyl acetate and butanol. Among these solvent fractions, kiwi rind extract of ethyl acetate (KRE-E) was observed to be the greatest. Immunofluorescence staining for the presence of AGEs in glycated collagen matrix showed that the glycation inhibitory effect of KRE-E and 5 mM of amnoguanidine were similar. Finally a phenolic compound of KRE-E was identified as quercetin-3-rhamnoside by employing HPLC. Recently using agricultural wastes as low cost source of potential raw material in cosmetics is an active research domain. Because these wastes give adventages of low cost and the possibility to reduce environmental problem caused by waste disposal.