원문정보
초록
영어
One of the major barriers for production of eukaryotic transgenic organism is very low or no expression of transgene. Transgene silencing is also a common phenomenon in Chlamydomonas reinhardtii as well as in higher plants. To develop the stable expression system of transgene in C. reinhardtii, we transformed a suppressor of RNA silencing, Cucumber mosaic virus 2b (CMV2b) and evaluated the RNA silencing activity in C. reinhardtii. Inhibition of Maa7 silencing was detected in CMV2bexpressing Maa7-IR44 strains, indicating that CMV2b suppressed siRNA pathways in C. reinhardtii as in higher plants. In addition, mRNA expression targeted for cleavage by miRNA was significantly higher in CMV2b-expressing strains, but increased accumulation of miRNA was not detected. Furthermore, reduction of AGO1, AGO2, AGO3, and DCL1 expression were detected in CMV2b-expressing strains. Taken together, our results suggest that CMV2b suppress both siRNA and miRNA pathways in C. reinhardtii, and CMV2b-expressing C. reinhardtii can be used as a host line for stable expression of transgene.