원문정보
초록
영어
Industrial microorganisms such as Saccharomyces cerevisiae and Corynebacterium glutamicum were engineered for assembly of designer minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and chimeric enzymes such as cellulase and agarase from different organisms. The chimeric enzymes fused with the dockerin domain of endoglucanase from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment different recalcitrant biomass including cellulose and agarose into commodity biofuels such as ethanol and biodiesel. The in vivo assembled minicellulosomes retained the synergistic effect for biomass hydrolysis. The minicellulosomes containing the cellulose binding domain was purified by crystalline cellulose affinity in a single step. This result indicates that a one-step processing of biomass in a consolidated bioprocessing configuration is carried out by recombinant microbial cells expressing functional minicellulosomes. The development of a more effective and high-yield cellulosic and/or agarosic biofuel fermentation process is required to bring about a necessary dramatic reduction of production costs. So this novel consolidated bioprocess will lead to more efficient commodity biofuel production from various sources of plant and marine biomass.