원문정보
Establishment of In vitro Detection System for iNOS Expression and the Verification of Suppressive Effect by Pine Needle Extract
초록
영어
This study was aimed to verify suppressive effect of pine-needle extract on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression. In order to evaluate suppressive effect on iNOS expression, RAW 264.7 cells were stably transfected using an iNOS promoterluciferase reporter plasmid yielding RAW 264.7/pGL2-NeomiNOS_ pro11 cells. Established in vitro detection system revealed to diminish LPS-induced iNOS expression by 0.1~500 μg/mL of saponin at the concentration-dependant manner. Pine needle extract also diminished LPS-induced iNOS expression to 92 and 88% at 500 and 50 μg/mL, respectively. These results suggest that the in vitro detection system developed here could be useful for the verification of suppressive materials on iNOS expression and pine needle extract could be used for the development of functional foods.
목차
1. 서론
2. 재료 및 방법
2.1. 시약 및 배지
2.2. iNOS 프로모터 유전자의 클로닝 및 리포터 플라스미드의 구축
2.3. Stable transfection
2.4. 적송잎 추출물 제조 및 LPS 유도 iNOS 발현 저해효과검증
3. 결과 및 고찰
3.1. iNOS 발현 검출 리포터 플라스미드의 구축
3.2. iNOS 발현 검출을 위한 세포주 확립
3.3. In vitro iNOS 발현 검출 시스템을 이용한 적송잎 추출물의 LPS 유도성 iNOS 발현 저해효과 검증
4. 결론
References