원문정보
초록
영어
The metastatic effect of Eupatorium japonicum extract (EJE) on MDA-MB-231 human breast cancer cells was investigated. MDA-MB-231 cells were treated with various concentrations of EJE (0, 5, 10 and 20 μg/mL). EJE inhibited cell migration, invasion and adhesion of MDA-MB-231 cells in dose-dependent manners. Gelatin zymography exhibited that EJE significantly down regulated secretion of matrix metalloproteinase (MMP)-9 and MMP-2. EJE decreased the protein levels of tissue inhibitor of metalloproteinase (TIMP)-1 but increased TIMP-2 levels. Additionally, EJE reduced the protein and mRNA levels of urokinase-type plasminogen activator (uPA), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule (ICAM). In several solvent fractions of EJE, the hexane fraction markedly decreased MDAMB- 231 cell migration. Thus, these finding suggest that EJE may be a potential antimetastatic agent, which can considerably inhibit the metastatic and invasive capacity of breast cancer cells.
목차
서론
재료 및 방법
세포주 및 실험재료
등골나물 추출물의 분리
세포배양
Total cell lysate
Medium concentration
Transwell migration, invasion and adhesion assay
Western blot analysis
Gelatin zymography
Real-time PCR
통계처리
결과 및 고찰
등골나물 추출물이 MDA-MB-231 세포의 이동 및 침윤에 미치는 영향
등골나물 추출물이 MDA-MB-231 세포의 MMPs, TIMPs 그리고 uPA의 분비에 미치는 영향
등골나물 추출물이 MDA-MB-231 세포의 VEGF 발현에 미치는 영향
등골나물 추출물이 MDA-MB-231 세포의 부착능에 미치는 영향
등골나물 추출물의 용매별 분획물이 MDA-MB-231 세포의 이동에 미치는 영향
요약
문헌