원문정보
Biosynthesis of Novel Polyhydroxyalkanoates Containing Various 4-Hydroxyalkanoates by Recombinant Microorganisms
피인용수 : 0건 (자료제공 : 네이버학술정보)
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
Bacterial strains and plasmids
Culture condition
Fermentation
Analytical procedure
Characterization of polymer by GC-MS analysis
Synthesis af 4- and 5-hydroxyalkanoates
Isolation and manipulation of DNA
Construction of genomic libraries af T. pfennigii
Identification and cloning of PHA-biosynthesis genes of T. pfennigii in PHA-negative mutant of A. eutrophus
Spectrometric determination of activity of PHA synthase
RESULTS AND DISCUSSION
Cloning of PHA -synthesis genes of T. pfennigii and heterologous expression in E. coli
Biosynthesis of PHA from various 4-hydroxyal-kanoates by recombinant P. putida
Biosynthesis of PHA containing 4HB and 4HHx as well as 3HB in recombinant Comamonas sp. FH-96
요약
ACKNOWLEDGEMENT
REFERENCES
INTRODUCTION
MATERIALS AND METHODS
Bacterial strains and plasmids
Culture condition
Fermentation
Analytical procedure
Characterization of polymer by GC-MS analysis
Synthesis af 4- and 5-hydroxyalkanoates
Isolation and manipulation of DNA
Construction of genomic libraries af T. pfennigii
Identification and cloning of PHA-biosynthesis genes of T. pfennigii in PHA-negative mutant of A. eutrophus
Spectrometric determination of activity of PHA synthase
RESULTS AND DISCUSSION
Cloning of PHA -synthesis genes of T. pfennigii and heterologous expression in E. coli
Biosynthesis of PHA from various 4-hydroxyal-kanoates by recombinant P. putida
Biosynthesis of PHA containing 4HB and 4HHx as well as 3HB in recombinant Comamonas sp. FH-96
요약
ACKNOWLEDGEMENT
REFERENCES
키워드
저자정보
참고문헌
자료제공 : 네이버학술정보