원문정보
Anti-diabetic effect of the exopolysaccharides (EPS) produced from Cordyceps sinensis on ob/ob mice
초록
영어
Anti-diabetic effect of the exopolysaccharides (EPS) produced from submerged mycelial culture of Cordyceps sinensis (Cs) was studiedin a type II diabetic animal model (C57BL/6J ob/ob). This study was designed to determine whether Cs-EPS improves clinical symptoms of type II diabetes in ob/ob mice. After Cs-EPS treatment at doses of 200 mg/kg body weight, the fasting blood glucose levels decreased by 47% after 7 weeks compared with those of the control mice. According to the oral glucose tolerance test, the glucose levels recovered its baseline after 120 min in Cs-EPS-treated mice, although the blood glucose levels increased significantly after 30 min. On the other hand, the control group (not-treated) did not recovered its initial level of glucose after 120 min. Furthermore, food intake, body weight, total plasma cholesterol and triglyceride concentrations in ob/ob mice treated with Cs-EPS were significantly decreased, compared with those in control ob/ob mice. Cs-EPS treatment increased significantly the plasma insulin level and the expression of leptin mRNA in adipose tissue of Cs-EPS-treated ob/ob mice. From these results, it is demonstrated that Cs-EPS could be effective for regulating normal blood glucose levels by increasing the amounts of plasma insulin and leptin expression in ob/ob mice, indicating that this compound could be a candidate material as a dietary supplement to control hyperglycemia in patients suffering from type II diabetes.
목차
1. Introduction
2. Materials andMethods
2.1. Microorganism
2.2. Inoculum preparation and culture condition
2.3. Preparation of EPS
2.4. Purification and content determination of EPS
2.5. Animals and breeding conditions
2.6. Experimental design
2.7. Oral glucose tolerance test
2.8. Analytical measurements
2.9. RNA isolation
2.10. RT-PCR analysis
2.11. Western blot analysis
2.12. Statistical analysis
3. Results and Discussion
3.1. Composition analysis of Cs-EPS
3.2. Effect of EPS on fasting blood glucose levels
3.3. Effect of EPS on oral glucose tolerance test
3.4. Effect of Cs-EPS on plasma insulin levels
3.5. Effect of EPS on plasma lipids and cholesterol
3.6. Effect of Cs-EPS on leptin expression
Acknowledgement
References