원문정보
FormⅠ Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from CO2-Fixing Chemoautotrophic bacterium, Aeromonas sp. strain JS-1: Purification and Properties
초록
영어
A new hydrogen-oxidizing bacterium, Aeromonas sp. strain JS-1, that can fix CO2 via the reductive pentose phosphate cycle (Calvin-Benson cycle) under chemoautotrophic conditions but not photoautotrophic conditions was isolated from fresh water. Strain JS-1 showed considerable CO2 fixation ability during continuous cultivation even at high CO2 concentration. Strain JS-1 used H2 and CO2 fixation as energy and carbon sources, respectively. Carbon dioxide fixation is carried out through the Calvin-Benson cycle, in which ribulose-1,5-bisphosphate carboxylase/ oxygenase (RubisCO) is the key enzyme. Hydrogen-oxidizing chemoautotrophic Aeromonas sp. strain JS-1 exhibited remarkedly strong RubisCO [EC 4.1.1.39] activity. RubisCO was purified as an L8S8-type hexadecamer with molecular mass of 560 kDa by gel filtration. The enzyme consisted of two different subunits eight large (56 kDa) and eight small (15 kDa), as demonstrated by SDS-PAGE. The specific activity of the purified enzyme was about 3.31 unit/mg and stable up to 45℃. The Km values for RuBP, CO2, and Mg2+ were estimated to be 0.25 mM, 5.2 mM and 0.91 mM, respectively.
목차
서론
재료 및 방법
균주 분리 및 배양
이산화탄소 고정경로 확인
RubisCO 활성 측정
단백질 정량
효소의 정제
전기영동
효소 화학적 성질
결과 및 고찰
수소산화 세균의 분리 및 동정
최적의 성장 조건
에너지원과 탄소원의 이용
Aeromonas sp. strain JS-1의 CO2 고정 경로
RubisCO의 분리 및 정제
RubisCO 분자량, subunits 구조
RubisCO의 일반적 성질
기질 특이성
결론
REFERENCES