원문정보
Anti-cancer Effects of Luteolin and Its Novel Mechanism in HepG2 Hepatocarcinoma Cell
초록
영어
In this study, we investigated the ability of luteolin, a plant derived flavonoid on hepatocarcinoma cell growth using HepG2 cell culture system. We found that luteolin increased the Smac/DIABLO releases, a mitochondrial protein that potentiates apoptosis. Luteolin also induced either transcriptional activity or expression of PPAR-gamma, a target of cancer growth that PPAR-gamma agonist sensitizes to apoptosis in certain cancer types. To find the possible upstream target molecules of PPAR-gamma activated by luteolin treatment, we used compound C, a specific inhibitor of AMP-activated protein kinase. Pre-treatment of Compound C significantly restored the activation or expression of PPAR-gamma stimulated by luteolin. This result indicated that AMPK signaling might be involved in the activation or expression of PPAR-gamma signaling pathway stimulated by luteolin. Moreover, we also found that luteolin inhibited the insulin-stimulated Akt phosphorylation as well as AICAR, a specific AMPK activator. These results propose that luteolin significantly induces cancer cell death through modulating survival signal pathways such as PPAR-gamma and Akt. AMPK signaling pathway may be an upstream regulator for survival signal pathways such as PPAR-gamma and Akt stimulated by luteolin.
목차
서론
재료 및 방법
세포배양 및 재료
단백질 추출 및 웨스턴 블롯팅
RT-PCR
PPAR감마 전사활성측정
Statistical analysis
결과
Smac/DIABLO의 발현에 대한 루테올린의 효과
PPARƔ의 발현 및 전사활성에 대한 루테올린의 촉진 및AMPK 억제제에 의한 이들 효과의 억제
루테올린과 AMPK활성제의 인슐린에 의한 Akt 인산화에 대한 억제 효과
고찰
결론
감사
REFERENCES