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루테올린의 간암세포 성장 억제효능 및 새로운 작용기전

원문정보

Anti-cancer Effects of Luteolin and Its Novel Mechanism in HepG2 Hepatocarcinoma Cell

황진택, 양혜정

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초록

영어

In this study, we investigated the ability of luteolin, a plant derived flavonoid on hepatocarcinoma cell growth using HepG2 cell culture system. We found that luteolin increased the Smac/DIABLO releases, a mitochondrial protein that potentiates apoptosis. Luteolin also induced either transcriptional activity or expression of PPAR-gamma, a target of cancer growth that PPAR-gamma agonist sensitizes to apoptosis in certain cancer types. To find the possible upstream target molecules of PPAR-gamma activated by luteolin treatment, we used compound C, a specific inhibitor of AMP-activated protein kinase. Pre-treatment of Compound C significantly restored the activation or expression of PPAR-gamma stimulated by luteolin. This result indicated that AMPK signaling might be involved in the activation or expression of PPAR-gamma signaling pathway stimulated by luteolin. Moreover, we also found that luteolin inhibited the insulin-stimulated Akt phosphorylation as well as AICAR, a specific AMPK activator. These results propose that luteolin significantly induces cancer cell death through modulating survival signal pathways such as PPAR-gamma and Akt. AMPK signaling pathway may be an upstream regulator for survival signal pathways such as PPAR-gamma and Akt stimulated by luteolin.

목차

Abstract
 서론
 재료 및 방법
  세포배양 및 재료
  단백질 추출 및 웨스턴 블롯팅
  RT-PCR
  PPAR감마 전사활성측정
  Statistical analysis
 결과
  Smac/DIABLO의 발현에 대한 루테올린의 효과
  PPARƔ의 발현 및 전사활성에 대한 루테올린의 촉진 및AMPK 억제제에 의한 이들 효과의 억제
  루테올린과 AMPK활성제의 인슐린에 의한 Akt 인산화에 대한 억제 효과
 고찰
 결론
 감사
 REFERENCES

저자정보

  • 황진택 Jin Taek Hwang. 한국식품연구원 바이오제론 연구단
  • 양혜정 Hye Jung Yang. 한국식품연구원 바이오제론 연구단

참고문헌

자료제공 : 네이버학술정보

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