원문정보
초록
영어
The adjuvant effects of Korean mistletoe lectin-C (KML-C) were investigated following the oral administration of KML-C with ovalbumin (OVA) as an antigen. Mice were orally immunized with OVA alone or admixed with various doses of KML-C or cholera toxin (CT), and the titer of OVA-specific antibody in the serum and mucosal secretions were determined. OVA+KML-C-treated mice showed high titers of IgA specific to CT in mucosal secretions. The antibody titers in the serum of OVA+KML-C-treated mice were comparable to those in the serum of OVA+CT-treated mice. When mice were immunized with OVA+KML-C or with CT alone and subsequently injected with OVA on the footpads after the primary immunization, they showed a more significant increase in delayed-type hypersensitivity reactions than when they were administered CT alone. These results suggest that KML-C is a potent immunoadjuvant that enhances both humoral and cellular immunity by the mucosal immune system.
목차
서론
재료 및 방법
시약 및 세포배양
한국산 겨우살이 렉틴의 분리
실험동물 및 면역
시료채취
항체 역가 측정
DTH test
통계처리
결과 및 고찰
OVA 특이적 IgA 형성에 미치는 역할
혈청 내 항원 OVA 특이적 항체의 역가에 미치는 영향
OVA 특이적 항체에 대한 Isotype 결정
지연성 과민반응(DTH) 유도에 미치는 영향
결론
감사의 글
문헌