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생리활성/영양

등골나물 추출물의 항염증 효과

원문정보

Anti-inflammatory Effect of Ethanol Extract from Eupatorium japonicum

이한나, 임도영, 임순성, 김종대, 윤정한

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Eupatorium japonicum belongs to a family of Asteraceae plants and flowers of E. japonicum have been consumed as a tea. In this study, we investigated whether E. japonicum extract inhibits lipopolysaccharide (LPS)-induced inflammatory responses in Raw264.7 macrophages. The cells were treated with various concentrations (0, 1, 2.5, 5, or 10 mg/L) of 70% ethanol extract from E. japonicum flowers (EJE) in Raw264.7 cells. LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production were inhibited by EJE up to 67% and 49% of these productions, respectively without any reduction of viable cell numbers. EJE reduced LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 proteins and their corresponding mRNA levels. Additionally, EJE decreased the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α mRNA. EJE was further fractionated with water, butanol, ethylacetate (EA), hexane, or methylene chloride (MC). Among the resulting five fractions, EA and MC, respectively from EJE significantly inhibited LPS-induced NO production (each inhibition rate was 85.3% of 10 mg/L EA fraction and 97.2% of 10 mg/L MC fraction) without significant cytotoxicity in Raw264.7 cells. These results indicate that EJE exhibits powerful effects of anti-inflammation and can be developed as a potential anti-inflammatory agent.

목차

Abstract
 서론
 재료 및 방법
  재료
  시료의 추출 및 분리
  세포배양
  NO와 PGE2 생성량과 세포생존율 측정
  염증 관련 단백질 수준
  염증성 사이토카인의 mRNA 발현 측정
  통계처리
 결과 및 고찰
  Raw264.7 세포에서 LPS에 의해 유도된 NO와 PGE2 생성에 대한 등골나물 에탄올추출물(EJE)의 효과
  LPS에 의해 유도된 iNOS, COX-2의 단백질과 mRNA 발현에 대한 EJE의 효과
  LPS에 의해 유도된 염증성 cytokine들의 mRNA발현에 대한 EJE의 효과
  Raw264.7 세포에서 LPS에 의해 유도된 EJE 분획물에 대한 NO억제 효과
 요약
 감사의 글
 문헌

저자정보

  • 이한나 Han Na Lee. 한림대학교 식품영양학과
  • 임도영 Do Young Lim. 한림대학교 식품영양학과
  • 임순성 Soon Sung Lim. 한림대학교 식품영양학과, 한림대학교 식의약품의 효능평가 및 기능성 소재개발센터
  • 김종대 Jong Dai Kim. 강원대학교 BT특성화학부 식품생명공학전공
  • 윤정한 Jung Han Yoon. 한림대학교 식품영양학과, 한림대학교 식의약품의 효능평가 및 기능성 소재개발센터

참고문헌

자료제공 : 네이버학술정보

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