원문정보
Comparison of Bio-ethanol Productivity Using Food Wastes by Various Culture Modes
초록
영어
In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 h-1), 6 h (0.07 h-1), 3 h (0.13 h-1). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 h-1.
목차
서론
재료 및 방법
음식물류폐기물의 전처리
음식물류폐기물의 당화
회분식 에탄올 발효
연속식 효모 고밀도 배양
감압/비감압식 fill & draw 동시 당화 발효에 의한 에탄올 생산
SFW 배지를 이용한 Fill & draw식 연속배양
환원당 농도 측정법
글루코스 농도 측정법
균체 농도 측정법
에탄올 농도 측정법
아밀라아제 활성 측정법
결과 및 고찰
회분식 배양에 의한 에탄올 발효
효모 고밀도 배양에 의한 연속식 에탄올 발효
감압/비감압식 fill & draw 동시 당화 발효
SFW 배지를 이용한 fill & draw식 연속 배양 발효
다양한 에탄올배양방식의 생산성 비교
결론
감사의 글
REFERENCES