원문정보
Simultaneous Determination of Tar Color Additives in Cold Syrups by Ion Pair-high Performance Liquid Chromatography
초록
영어
A simple and efficient analytical method for the simultaneous determination of seven tar color additives was developed using ion pair high performance liquid chromatography. The conditions for HPLC analysis were as follows: column, μ-Bondapak C18 (10 μm, 300 × 3.9 mm i.d.); gradient mobile phase, 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrile-methanol (65:25:10) as a mobile for fraction A and 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrilemethanol (40:50:10) as a mobile for fraction B; flow rate, 1.0 mL/ min; detection wavelength, 254/520/620 nm. We could attain to the detection limits as 0.01~0.05 μg/mL (254 nm) and 0.005~0.01 μg/mL (520 nm) for six red tar color additives, and 0.05 μg/mL (254 nm) and 0.002 μg/mL (620 nm) for Fast green FCF. This analytical method was applicable to determine the tar color additives contained in several commercial cold syrups.
목차
서론
재료 및 방법
시약 및 기기
HPLC 측정조건
표준용액의 제조
Assay validation
시료 전처리
결과 및 고찰
컬럼의 선택
파장의 선택
이동상의 조성이 분리능에 미치는 영향
검출한계 및 정량한계
시판 감기약 시럽 약물 중 타르색소 검출 및 함량 측정
분석법 validation
회수율 실험
결론
감사
REFERENCES