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Conjugation of flagellin with Capsular Polysaccharides of Streptococcus pneumoniae Type 19A

원문정보

Sang-il YUN, Hwa-Ja RYU, Hee Kyoung KANG, Eun Seong SEO, Doman KIM

한국생물공학회 한국생물공학회 학술대회 2010 추계학술대회 및 국제심포지움 2010.10 pp.276-276
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초록

영어

The Gram-positive pathogenic bacterium Streptococcus pneumoniae is cause of pneumonia, meningitis, otitis media and sepsis [1]. In particular, more than 90% pneumonia patients catch a pneumonia disease due to S. pneumoniae. Its elements of the capsular polysaccharide, C substance, F antigen and M protein are the antigens.
That of the capsular polysaccharide is used to product of vaccines. 91 kinds of capsular polysaccharides types are known until now [2]. In this study, we obtained capsular polysaccharides from S. pneuminae type 19A (CPS19A). CPS19A was purified by chemical treatment and ethanol precipitation. The size of capsular polysaccharide was reduced by acid hydrolysis and then hydrolysate of CPS19A was purified by ethanol precipitation and gel permeation chromatography. Different size of CPS19A was carried out conjugation with the flagellin. The conjugation result was identified by thin layer chromatography, SDS-PAGE and western blot analysis.

키워드

저자정보

  • Sang-il YUN School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju, 500-757.
  • Hwa-Ja RYU School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju, 500-757.
  • Hee Kyoung KANG School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Gwangju, 500-757.
  • Eun Seong SEO School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Gwangju, 500-757.
  • Doman KIM School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju, 500-757.

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