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Purification of Capsular Polysaccharide Produced by Streptococcus pneumoniae Serotype 19A

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Streptococcus pneumoniae causes many types of pneumococcal infection; especially, the incidence of pneumococcal disease which is the highest in infants under 2 years and in people over 60 years of age.
Capsular polysaccharides (CPS) are the primary basis for pathogenicity, antigenic and serotypes. To purify CPS for producing vaccine, we developed a simple process including four steps. Initially, the cells were collected by centrifugation. And they were lysed using detergent.
Second, the proteins and cell debris were precipitated and removed by the addition of acetic acid. Third, pure CPS was prepared by ethanol concentration fractionation (50~80%, v/v) at pH 7.0. Finally, the purified CPS was further dialyzed against dH2O. This process would save cost, time and labor in comparison with current methods developed by others. Thus, this modified process would be suitable for preparation of conjugated CPS as vaccine to reduce the pneumococcal diseases.

저자정보

  • Bui Nguyet MINH Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University.
  • Seung-Jin JUNG Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University.
  • Sang-Il YOON School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University.
  • Eun-Seong SEO School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University.
  • Doman KIM Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University.

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