원문정보
초록
영어
Streptococcus pneumoniae causes many types of pneumococcal infection; especially, the incidence of pneumococcal disease which is the highest in infants under 2 years and in people over 60 years of age.
Capsular polysaccharides (CPS) are the primary basis for pathogenicity, antigenic and serotypes. To purify CPS for producing vaccine, we developed a simple process including four steps. Initially, the cells were collected by centrifugation. And they were lysed using detergent.
Second, the proteins and cell debris were precipitated and removed by the addition of acetic acid. Third, pure CPS was prepared by ethanol concentration fractionation (50~80%, v/v) at pH 7.0. Finally, the purified CPS was further dialyzed against dH2O. This process would save cost, time and labor in comparison with current methods developed by others. Thus, this modified process would be suitable for preparation of conjugated CPS as vaccine to reduce the pneumococcal diseases.