원문정보
초록
영어
Prostate cancer is the second most common type of cancer afflicting American males, and yet, little is known about which cell types within the prostate are the targets of tumorigenic transformation. Recent studies support the hypothesis that cancer stem cells (CSCs) are responsible for tumor initiation and formation. The purpose of this study is to investigate prostate CSCs and discover their markers with human prostate cancer cell line DU145. DU145 was separated into CD44+ and CD44- cells using MACS. CD44 is proposed to mark a population of prostate CSCs even though it is still controversial. In soft agar colony forming assay, CD44+ cells formed much more colonies compared to CD44- cells, which means CD44+ cells possess cancer stem cell characteristics. Thus, we focused on proteins that were differentially expressed between CD44+ and CD44- cells. The protein expression was analyzed by two-dimensional gel electrophoresis (2-DE) and the differentially expressed proteins were identified by LC MS/MS. We acquired protein A and B that are known to be related to CSCs' biological mechanism. This results show that these proteins may constitute a useful biomarker for the diagnosis of prostate cancer.
Moreover, these proteins would contribute to better understanding the mechanism of prostate CSCs.