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영어

Type 2 diabetes (T2D) is characterized by hyperglycemia, which is caused by insulin resistance and relative insulin deficiency. Type 2 diabetic patients have higher risk of acute liver failure than non-diabetic patients. In this study, we investigated the possible hepatic protective effects of vitamin A, as an antioxidant, in the T2D rat model. Male Sprague-Dawley rats (150-160 g, n=21) were divided into 3 groups: normal group, diabetic group and vitamin A-treated diabetic group. To confirm liver injury before and after vitamin A treatment, serum levels of hepatic enzyme markers (GOT and GPT) and the liver-to-body weight ratio were measured for 4 weeks. As a result of these experiments, we found that vitamin A was effective in protecting the T2D rat model from liver injury. Using two-dimensional gel electrophoresis (2-DE), we have compared differences of rat liver proteome. Differentially expressed proteins in the diabetes group compared to the normal group and in the vitamin A-treated diabetes group compared to the diabetic group were identified using ESI-Q-TOF MS/MS analyses. Therefore, these proteins may play a key role in the development and recovery of liver injury by vitamin A-treatment in the T2D rat model.