earticle

영어

Planar bilayer lipid membranes (BLMs) are primary tools in studying ion channels that working lipid bilayer and bio screening. Painting methods, introduced by Muller, are used widely but the method has several drawbacks of needed high skilled operator and lack of stability that assemble it inefficient. For this reason Mueller method has critical drawbacks as substituted by patch clamp method, another gold standard method to study ion channels in live cell. Consequently, Mueller method to assemble lipid bilayer has been studied for increasing stability, lessen intensive labor and decreasing degree of skill required. First, we use high melting point lipid solution which 2:8 squalene and hexadecane mixture. The lipid solution freezes at ~14°C. The lipid solution was deposited a small aperture and frozen before using at experiment. The membrane precursor can be stored freezing and enabling transportation. This membrane precursor can be transported to other place and thawed where a membrane is needed. However, using high melting point lipid solution method has a low success rate (~30%) and various thinning out time. To overcome the problem, pin tool is used for exact volume control in previous work. Despite increased precision of deposition, several problems remained. To resolve problems we used PDMS gasket instead of conventional hydrophobic films.
PDMS gasket extracts organic solvent, promoting lipid bilayer formation. This characteristic advanced optimization and at the same time reduced the membrane formation time to ~30 minutes.
Membranes were created timely in a controlled. Adapt to automated array system. An array of lipid bilayer membrane can be developed for the potential use of high-throughput ion channel screening.
To exhibit high-throughput measurement of ion channels use an analog switch, enabling sequential measurement of each membrane using just one amplifier. Membranes measured using this switch were shown identical to those in the conventional system. We will show a number of potential applications using our membrane system. BLMs can be used for high-throughput screening.