원문정보
초록
영어
Various methods have been developed to concentrate bacteria from the raw sample by their coagulations and sedimentations. However, these methods did not prefer to low concentration bacteria in the real world applications. In this study, we developed simple concentration method of gram negative and positive bacteria (Escherichia coli and Bacillus cereus, respectively). High concentrated bacteria (gram negative and positive) were well isolated using hydrophilic surface under salty condition (pH4, 100mM of phosphate) whereas the adsorption efficiencies of bacteria were dramatically decreased in case of low concentrated bacteria. We optimized the bacteria adsorption on hydrophilic surface using aquatic chemistry. The optimal conditions were determined with PEG and divalent cations for E. coli and with divalent anions for B. cereus. The adsorption rate increased up to 50% when low concentration bacteria less than 102c.f.u were applied with optimal conditions. As a result, this method could be directly applied to detect E. coli in low concentration in beef sample [101c.f.u/g].
Acknowledgment: This subject is supported by Ministry of Environment of the Republic of Korea as “The Eco-Technopia 21 project” and by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No.M10755160002-20100020657))