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바이오센서 및 바이오칩

Human bitter taste receptor functionalized-swCNT-FETas a human tongue like-artificial taste sensor

원문정보

Hyun Seok SONG, Sang Hun LEE, Hyejun JIN, Sae Ryun AHN, Eunghun HONG, Eun Hae OH, Tai Hyun PARK

한국생물공학회 한국생물공학회 학술대회 2010 추계학술대회 및 국제심포지움 2010.10 pp.252-252
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Artificial taste sensors, so called ‘electronic tongues’, are normally used in food and beverage industry. Usually those electronic tongues utilize synthetic materials but cannot mimic the biological features of the human tongue. To overcome these limitations, we applied biological human taste receptor to the development of artificial taste sensor. Here, we demonstrated human bitter taste receptor, hTAS2R38, produced from E.
coli-functionalized single-walled carbon nanotube (swCNT)-field effect transistor (FET) can detect target tastant with high selectivity and sencitivity. Each taster (PAV) and non-taster (AVI) haprotype of human bitter taste receptor genes, hTAS2R38, were cloned into bacterial expression vector and expressed in E. coli at high-level. The receptors were then immobilized on swCNT-FET sensor platform and stimulated by various tastants. The taster haplotype (PAV) of hTAS2R38 on swCNT-FET responded to bitterness compounds, phenylthiocarbamide (PTC) and propylthiouracil (PROP), with high sensitivity up to concentration as low as 100 fM but non-taster haplotype (AVI) did not. This artificial taste sensor showed very similar to performance with human taste system.

키워드

저자정보

  • Hyun Seok SONG School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Sang Hun LEE School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Hyejun JIN Department of Physics and Astronomy, Seoul National University, Seoul 151-742 Korea.
  • Eun Hae OH School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Sae Ryun AHN School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Eunghun HONG Department of Physics and Astronomy, Seoul National University, Seoul 151-742 Korea.
  • Tai Hyun PARK School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.

참고문헌

자료제공 : 네이버학술정보

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