earticle

영어

Total 7 PCBs antigen have been produced and used for the antibody generation and validation. And 6 polyclonal antibodies against PCBs have been produced G1-1/G1-2/G2-1/G2-2/G2-3/G2-4), 19 monoclonal antibodies {1F7, 2C7,5A6(G2-1), 2A11, 3A11, 4D7, 22G7(G2-2), 1G6, 10F9, 12G5(G2-3), 1E7, 2A10, 3H4, 4F10, 5A9, 5C2, 6F1, 6H3, 7C4(G2-4)} against PCBs are developed. 11 clones out of 19 are massively produced. 8 clones have been conjugated with gold. In final, 2 clones 2A11 (G2-2), 2A10(G2-4) have been selected for the Biosensor as a result of validation test. It is expected that the cost and time for measuring the transformers’ PCB by the developed antibodies would be more competitive and swift than by GC-MC or GC-ECD. Also the more effective Biosensor would be developed if the additional validation test is performed and PCBs detect test is supplemented.