원문정보
초록
영어
Plant cells have several advantages as a host for the production of therapeutic proteins, such as low production cost and safety from animal pathogens.
Recently, many therapeutic proteins are produced by using transgenic plant cell cultures. Transgenic rice cell cultures (Oryza sativa L.) with inducible RAmy3D promoter have been used to express recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig), a soluble fusion protein used for the treatment of rheumatoid arthritis. Induction of the RAmy3D promoter is strongly performed by sugar starvation and the transgenic rice cells secrete hCTLA4Ig into the medium. Sodium butyrate (NaBu) has been used to enhance the production of foreign proteins in Chinese hamster ovary (CHO) cell cultures due to its harmlessness, easy manipulation and cost-effectiveness. However, it may also inhibit cell growth and induce cellular apoptosis. In this study, for the enhanced production of hCTLA4Ig, effects of NaBu on cell growth and protein production were investigated.
Feeding concentration (0, 0.5, 1, 5 mM) of NaBu as well as the supplementation of additional sodium pyruvate (87 mM) as an energy source were optimized and studied at induction phase. In conclusion, the production of hCTLA4Ig could be significantly enhanced by adding 1 mM NaBu with additional energy source in transgenic rice cell cultures.