earticle

영어

Recently, disposable bioreactors have been increasingly incorporated into preclinical, clinical, and production-scale biotechnological facilities. However, there are few studies regarding their suitability for the production of pharmaceutical proteins in transgenic plant cell cultures. Plant cell culture have been regarded as a potential alternative production system for biopharmaceuticals. In this study, two kinds of disposable bioreactors were applied to transgenic rice cell cultures for the production of recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) and they were compared with 5-L stirred tank bioreactor system. The first one is a modified wave-mixed bag with a fritted sparger, and the second is an airlift disposable bioreactor. In both systems, fed-batch culture method was used for the efficient production of hCTLA4Ig by feeding concentrated amino acids.
Dissolved oxygen level was regulated at 30% by supplementing pure oxygen with solenoid valve during the run. The results from wave-mixed and airlift disposable bioreactor showed similar maximum cell density (12.6 vs. 11.9 g DCW/L), doubling time (5.03 vs. 4.84 day) and maximum hCTLA4Ig concentration (44.5 vs. 43.7 mg/L).
However, the time needed for obtaining maximum hCTLA4Ig level was much longer in airlift disposable bioreactor (26 vs. 34 days). With higher aeration rate (0.2 vvm), 1.7-fold higher specific productivity (0.355 mg/g DCW/day) was obtained. These results show that pnumatically driven disposable bioreactor system is better for the production of recombinant proteins from plant cell cultures.