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영어

The comet assay can theoretically be applied to every type of eukaryotic cell, including plant cells. Experiments were conducted to study the genotoxic effects of radiations on the genome integrity, particularly in Tradescantia [1]. Tradescantia tests are very useful tools for screening the mutagenic potential in the environment [2-3]. This study was designed to assess the genotoxicity of ionizing radiation using Tradescnatia-comet assay. Tradescnatia leaf samples were exposed to 0.1-50Gy. As an estimate of DNA migration, the length of the comet tail was recorded versus its head diameter under different conditions. In the non-irradiated sample the tails can be seen, but the DNA comets are short. The comet moment parameter which integrates both the morphometric parameter and the densitometric one may better reflect the amount of DNA which has been displaced from the nucleus. The comet assay has demonstrated its sensitivity as a technique for the evaluation of DNA damage among a variety of cell types, induced by a variety of physical and chemical agents [4]. The potential applications of the comet assay in such areas as genotoxicity, DNA repair, environmental biomonitoring and human monitoring are almost unlimited.