원문정보
초록
영어
Rock bream iridovirus (RBIV) have occurred frequently among cultured marine fish species in Southeast Asia (including S. Korea) and iridoviral disease has caused severe systemic disease with high mortality. The major capsid protein of RBIV is known to have a major effect on viral infection.
In order to develop the edible vaccine against RBIV, MCP gene with KDEL endoplasmic reticulum retention signal was synthesized based on rice optimized codon usage (sMCP) by overlap PCR. The synthetic fragments, sMCP was inserted into a plant expression vector containing sugar starvation inducible promoter, RAmy3D, and introduced into the rice celli by bombardment-mediated transformation. The stable integration and transcriptional expression of the sMCP were confirmed by genomic DNA PCR, Northern blot analysis. Accumulation and production of major capsid protein in endoplasmic reticulum during sugar starvation in suspension-cultured cells was confirmed by Western blot analysis. These results suggest that transgenic suspension-cultured rice cells have the potential to be used as a edible vaccine against RBIV (This study was supported by Technology Development Program (108166-03-1-HD110) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea).