earticle

영어

Perfusion culture of mammalian cells including embryonic stem cell (ESC) is attractive, given that culture environment can be maintained consistently. Cell membrane motility deformed by fluidic stress presents aspects of adhesion, stretching and migration belonging to intercellular molecular dynamics. Consequently, cell feature and motility have been carried on cellular physicochemistry as key characteristic indices. We suggest Lf as a new parameter, which represents the free peripheral membrane length without attaching with other cells to the each cell's peripheral length. Statistical analysis demonstrates that Lf have positive correlation with cell roundness(Rn), area of cytoplasm(Ac) but a negative correlation with cellular area(Ar).