원문정보
초록
영어
We investigated the effects of Aurintricarboxylic Acid (ATA) on Chinese Hamaster Ovary (CHO) DG44 cell growth in serum free media with controlled calcium concentration, measuring the cell aggregation rate with the coulter counter. In previous studies, high levels of extracellular calcium ion (12.5 mg/L) showed to cause decreased cell density and high aggregation to CHO DG44 cells in serum-free media.
Cell aggregation during cell culture causes hindrance of nutrient and oxygen transfer to the cell, leading to inhibition of cell growth. We observed increased cell growth and decreased cell aggregation when we added ATA in the calcium controlled medium. Exhibited effects of ATA on cell aggregation particularly appeared in high-calcium levels. In commercial serum-free media that cannot control calcium concentrations, ATA is a possible solution that may solve the cell aggregation problem in high-calcium conditions.