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영어

Porcine epidemic diarrhea virus (PEDV) is a Coronavirus and a causative agent for diarrhea in pigs, particularly in neonates. The propagation of the PEDV in cell culture was developed in 1988. Porcine epidemic diarrhea disease vaccine was produced from Vero cells by using lively attenuated virus strain[1]. The PEDV strain of PED-SM was tested in this study. The optimal MOI, infection time, and harvest time of PED-SM were 0.1, 72 hr of post-inoculation, and 24 hr of post-infection. With 0.1 MOI, PED-SM showed not only the highest virus production rate, but also highest cell death rate. Therefore, the maintenance of high cell density for long time is keenly needed for the high virus titer. A semi-perfusion culture of Vero cells was tried for the high cell density culture. The medium change with 10% FBS supplemented medium showed better cell growth than that with other concentration of FBS. However, the virus titer with medium change was lower than that with no medium change. This maybe caused by the depletion of nutrients in virus production medium due to the high cell density in semiperfusion culture. Accordingly, additional nutrients supplement maybe needed in the virus production step as well as in the cell culture step.