earticle

영어

Mesenchymal stem cells (MSCs) have a great potential in cell-based therapies because of their multipotency and simple methods for in vitro manipulation. However, during in vitro manipulation, MSCs have aging and loss their multipotency and proliferation capability. Previous studies shown that calorie restriction (CR) induces proliferation of MSCs and diminishes apoptosis. Therefore, we observed the effect of low glucose in MSC in vitro. Proliferation level in the low glucose (LG, 1.375 mM) condition was compared with that in the normal glucose (NG, 5.5 mM) condition. We performed comparative studies of population doubling, β-galactosidase activity, ROS generation and differentiation capacity (osteocyte, adipocyte) in NG and LG conditions. We compared protein expressions between NG and LG conditions, several proteins were up-regulated or down-regulated in glucose restriction condition, LG condition. These results will be expected to provide a powerful tool for long-term and large scale culture of stem cells.