earticle

영어

Mesenchymal stem cells (MSCs) derived from human umbilical cord blood (hUCB) represent encouraging candidates for the development of future cellular therapy strategies. MSCs have been found to have pluripotent which is able to differentiate into various tissues. One of the primary limitations in our study of the biology of human MSCs is the absence of prospective markers required for the monitoring of lineage-specific differentiation. hUCB-derived MSCs have been found to have significantly greater osteogenic potential. In this study, we focused on protein expression changes during osteogenic differentiation of hUCB-MSCs (n=3). And we analyzed the protein expression inherent to osteogenic differentiation by proteomic research including two-dimensional gel electrophoresis, ESI-Q-TOF, and Western blotting.
Eleven differentially expressed proteins including β-actin, pyridoxal phosphatase, PGAM1, VBP1, Hsp27 and HSPC231 were confirmed between the two groups (before and after differentiation). These might also be proved as useful cytosolic biomarkers for osteogenesis, and might be employed in quality control of osteoblasts in cell-therapy applications.