earticle

영어

Hyperglycemia can become severe and lead to serious complications, such as microvascular complications of diabetes and diabetic nephropathy (DN). Recent studies have focused on glomerular dysfunction found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggests the involvement of the tubulointerstitium. However, the etiology of the tubulointerstitial pathology in DN is not yet fully rnderstood. In this study, to understand the DN pathways, we planned to produce a 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell under 5 mM and 25 mM glucose, which correspond to normoglycemia and hyperglycemia conditions, respectively. The differences in the expression of HK-2 cell cellular proteins under hyperglycemia condition were identified via ESI-Q-TOF MS/MS and confirmed by Western blotting; enolase 1 (up-regulated) and lactate dehydrogenase (down-regulated). The regulation of these proteins will contribute to understanding DN mechanism through the glycolysis metabolic pathways in high glucose stimulated HK-2 cells.